The P3 Primary Cell 4D-NucleofectorTM X Kit L is one of our five 4D-NucleofectorTM Kits suited for transfecting primary cells in the 100 μL NucleocuvetteTM Vessel format when working with the 4D-NucleofectorTM X Unit. This kit has been determined to be optimal for transfecting following cell types: ' B cell (human) CD34+ cell (human) Chondrocyte (human) DC (human) DC (mouse), mature ES (human), H9 ES (mouse) Hepatocyte (human) HMEC (human) Keratinocyte (human) Macrophage (human) Monocyte (human) Neuron (rat), brain Hi/Cx NHBE (human) RPE (human) T cell (human), stim. T cell (mouse), unstim. The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.