The total soluble Neuropilin-1 (NRP1) immunoassay is a 4 hour, 96-well sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of free and ligand-bound soluble Neuropilin-1 in human serum and plasma. The assay employs human serum-based standards to ensure the measurement of biologically reliable data.

NRP1 measurements in urine, cell-culture supernatants and non-human samples did not undergo a full validation according to FDA/ICH/EMEA guidelines. However, our performance check suggests that these matrices can be measured with this ELISA. For more information please see our validation data file.

The total soluble Neuropilin-1 ELISA kit uses highly purified, epitope mapped antibodies. The detection antibody used in the total soluble Neuropilin-1 ELISA kit binds to a linear epitope close to the N-terminus in the CUB1 domain of NRP1 and the polyclonal coating antibody recognizes multiple linear epitopes distributed over the entire Neuropilin-1 molecule.